Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University

自治医科大学 分子病態治療研究センター
再生医学研究部

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私立大学戦略的研究基盤形成支援事業「マウスからヒトへ」のリンク


私立大学戦略的研究基盤形成支援事業「マウスからヒトへ」のリンク






研究業績(抜粋)
Year Article Link
2017
Kobayashi E, Hanazono Y, Kunita S. Swine used in the medical university-overview of 20 years of experience. Exp Anim. 2017 Oct 7. doi: 10.1538/expanim.17-0086.
Abe T, Matsuoka Y. Nagao Y. Sonoda Y, and Hanazono Y. CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep. Int J Hematol. 2017 Nov;106(5):631-637. DOI 10.1007/s12185-017-2290-5.
Ohmori T, Nagao Y, Mizukami H, Sakata A, Muramatsu SI, Ozawa K, Tominaga SI, Hanazono Y, Nishimura S, Nureki O, and Sakata Y. CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia B mice. Sci Rep. 2017 Jun 23;7(1):4159. doi: 10.1038/s41598-017-04625-5.
Yamaguchi T, Sato H, Kato-Itoh M, Goto T, Hara H, Sanbo M, Mizuno N, Kobayashi T, Yanagida A, Umino A, Ota Y, Hamanaka S, Masaki H, Rashid ST, Hirabayashi M, Nakauchi H. Interspecies organogenesis generates autologous functional islets. Nature. 2017 Feb 9;542(7640):191-196. doi: 10.1038/nature21070.
Cho GS, Lee DI, Tampakakis E, Murphy S, Andersen P, Uosaki H, Chelko S, Chakir K, Hong I, Seo K, Chen HV, Chen X, Basso C, Houser SR, Tomaselli GF, O' Rourke B, Judge DP, Kass DA, and Kown C. Neonatal transplantation confers maturation of PSC-derived cardiomyocytes conducive to modeling cardiomyopathy. Cell Rep. 2017 Jan 10; 18(2): 571-582.
2016
Abe T, Kono S, Ohnuki T, Hishikawa S, Kunita S, and Hanazono Y. A swine model of acute thrombocytopenia with prolonged bleeding time produced by busulfan. Exp Anim. 2016 Nov 1; 65(4): 345-351.
Arai Y, Fukukawa H, Atozi T, Matsumoto S, Hanazono Y, Nagashima H, and Ohgane J. Ultra-deep bisulfite sequencing to detect specific DNA methylation patterns of minor cell types in heterogenous cell populations: an example of the pituitary tissue. PLoS One. 2016 Jan 11; 11(1):e0146498.
2014 Abe T, Hanazono Y, and Nagao Y. A long-term follow-up study on the engraftment of human hematopoietic stem cells in sheep. Exp Anim. 2014 Jul 22; 63(4): 475-481
Mizukami Y, Abe T, Shibata H, Makimura Y, Fujishiro SH, Yanase K, Hishikawa S, Kobayashi E, and Hanazono Y. MHC-matched induced pluripotent stem cells can attenuate cellular and humoral immune responses but are still susceptible to innate immunity. PLoS One. 2014 Jun 13; 9(6):e98319.
2013 Watanabe M, Nakano K, Matsunari H, Matsuda T, Maehara M, Kanai T, Kobayashi M, Matsumura Y, Sakai R, Kuramoto M, Hayashida G, Asano Y, Takayanagi S, Arai Y, Umeyama K, Nagaya M, Hanazono Y, Nagashima H. Generation of interleukin-2 receptor gamma gene knockout pigs from somatic cells genetically modified by zinc finger nuclease-encoding mRNA. PLoS One. 2013 Oct 9;8(10)e76478.
Arai Y, Ohgane J, Fujishiro SH, Nakano K, Matsunari H, Watanabe M, Umeyama K, Azuma D, Uchida N, Sakamoto N, Makino T, Yagi S, Shiota K, Hanazono Y, Nagashima H. DNA methylation profiles provide a viable index for porcine pluripotent stem cells. Genesis. 2013 Aug 2. doi: 10.1002/dvg.22423.
Hanazono Y. Stem cell research using large animals. Rinsho Ketsueki. 2013 Apr;54(4)329-335
Nakano K, Watanabe M, Matsunari H, Matsuda T, Honda K, Maehara M, Kanai T, Hayashida G, Kobayashi M, Kuramoto M, Arai Y, Umeyama K, Fujishiro SH, Mizukami Y, Nagaya M, Hanazono Y, Nagashima H. Generating porcine chimeras using inner cell mass cells and parthenogenetic preimplantation embryos. PLoS One. 2013 Apr 23;8(4)e61900.
Fujishiro SH, Nakano K, Mizukami Y, Azami T, Arai Y, Matsunari H, Ishino R, Nishimura T, Watanabe M, Abe T, Furukawa Y, Umeyama K, Yamanaka S, Ema M, Nagashima H, Hanazono Y. Generation of naive-like porcine-induced pluripotent stem cells capable of contributing to embryonic and fetal development.  Stem Cells Dev. 2013 Feb 1;22(3)473-482.
2012 Maehara M, Matsunari H, Honda K, Nakano K, Takeuchi Y, Kanai T, Matsuda T, Matsumura Y, Hagiwara Y, Sasayama N, Shirasu A, Takahashi M, Watanabe M, Umeyama K, Hanazono Y, Nagashima H. Hollow fiber vitrification provides a novel method for cryopreserving in vitro maturation/fertilization-derived porcine embryos. Biol Reprod. 2012 Dec 6;87(6)133.
Abe T, Masuda S, Tanaka Y, Nitta S, Kitano Y, Hayashi S, Hanazono Y, Nagao Y. Maternal administration of busulfan before in utero transplantation of human hematopoietic stem cells enhances engraftments in sheep. Exp Hematol. 2012 Jun;40(6)436-444.
2011 Hanazono Y. Generation of high quality induced pluripotent stem cells. Seikagaku. 2011 Nov; 83(11)1060-1063.
Masuda S, Hayashi S, Ageyama N, Shibata H, Abe T, Nagao Y, Hanazono Y. Migration of cells from the yolk sac to hematopoietic tissues after in utero transplantation of early and mid gestation canine fetuses. Transplantation. 2011 Jul 27;92(2):e5-6.
Masuda S, Hanazono Y. Induced pluripotent stem cells in long-QT syndrome. N Engl J Med. 2011 Jan 13;364(2):181.
Abe T, Masuda S, Ban H, Hayashi S, Ueda Y, Inoue M, Hasegawa M, Nagao Y, Hanazono Y. Ex vivo expansion of human HSCs with Sendai virus vector expressing HoxB4 assessed by sheep in utero transplantation. Exp Hematol. 2011 Jan;39(1):47-54.
2010 Tanaka Y, Masuda S, Abe T, Hayashi S, Kitano Y, Nagao Y, Hanazono Y. Intravascular route is not superior to an intraperitoneal route for in utero transplantation of human hematopoietic stem cells and engraftment in sheep. Transplantation. 2010 Aug 27;90(4) 462-463.
2009 Masuda S, Kumano K, Suzuki T, Tomita T, Iwatsubo T, Natsugari H, Tojo A, Shibutani M, Mitsumori K, Hanazono Y, Ogawa S, Kurokawa M, Chiba S. Dual antitumor mechanisms of Notch signaling inhibitor in a T-cell acute lymphoblastic leukemia xenograft model. Cancer Sci. 2009 Dec;100(12) 2444-2450
Masuda S, Ageyama N, Shibata H, Obara Y, Ikeda T, Takeuchi K, Ueda Y, Ozawa K, Hanazono Y. Cotransplantation with MSCs improves engraftment of HSCs after autologous intra-bone marrow transplantation in nonhuman primates. Exp Hematol. 2009 Oct;37(10) 1250-1257.e1
Tanaka Y, Ikeda T, Kishi Y, Masuda S, Shibata H, Takeuchi K, Komura M, Iwanaka T, Muramatsu S, Kondo Y, Takahashi K, Yamanaka S, Hanazono Y. ERas is expressed in primate embryonic stem cells but not related to tumorigenesis. Cell Transplant. 2009;18(4) 381-389
Nagao Y, Abe T, Hasegawa H, Tanaka Y, Sasaki K, Kitano Y, Hayashi S, Hanazono Y. Improved efficacy and safety of in utero cell transplantation in sheep using an ultrasound-guided method. Cloning Stem Cells. 2009 Jun;11(2) 281-285
2008 Kishi Y, Tanaka Y, Shibata H, Nakamura S, Takeuchi K, Masuda S, Ikeda T,Muramatsu S, Hanazono Y. Variation in the incidence of teratomas afterthe transplantation of non-human primate ES cells into immunodeficientmice. Cell Transplant 2008;17:1095-1102
Kishi Y, Inoue M, Tanaka Y, Shibata H, Masuda S, Ikeda T, Hasegawa M, Hanazono Y. Knockout Serum Replacement (KSR) has a suppressive effect on Sendai virus-mediated transduction of cynomolgus ES cells. Cloning Stem Cells. September 2008, 10(3): 307-312.
Tanaka Y, Nakamura S, Shibata H, Kishi Y, Ikeda T, Masuda S, Sasaki K, Abe T, Hayashi S, Kitano Y, Nagao Y, Hanazono Y. Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation. Stem Cells Dev. 2008 Apr;17(2):367-81.
2006 Shibata H, Ageyama N, Tanaka Y, Kishi Y, Sasaki K, Nakamura S, Muramatsu S, Hayashi S, Kitano Y, Terao K, Hanazono Y. Improved safety of hematopoietic transplantation with monkey ES cells in the allogeneic setting. Stem Cells 2006 Jun;24(6):1450-1457. 
Ageyama N, Hanazono Y, Shibata H, Ono F, Nagashima T, Ueda Y, Yoshikawa Y, Hasegawa M, Ozawa K, Terao K. Prevention of immune responses to human erythropoietin in cynomolgus monkeys (Macaca fascicularis). J Vet Med Sci 2006 May;68(5):507-510. 
Asano T, Shibata H, Hanazono Y. Use of simian immunodeficiency virus vectors for simian embryonic stem cells. Methods Mol Biol 2006 Feb;329:295-303. 
Asano T, Sasaki K, Kitano Y, Terao K, Hanazono Y. In vivo tumor formation from primate embryonic stem cells. Methods Mol Biol 2006 Feb;329:459-467. 
2005 Ageyama N, Hanazono Y, Shibata H, Ono F, Ogawa H, Nagashima N, Ueda Y, Yoshikawa Y, Hasegawa M, Ozawa K, Terao K. Safe and efficient collection of cytokine-mobilized peripheral blood cells from cynomolgus monkeys (Macaca fascicularis) with human newborn-equivalent body weights. Exp Anim 2005;54(5):421-428. 
Yoshioka T, Ageyama N, Shibata H, Yasu T, Misawa Y, Takeuchi K, Matsui K, Yamamoto K, Terao K, Shimada K, Ikeda U, Ozawa K, Hanazono, Y. Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34+ stem cells in a nonhuman primate model. Stem Cells 2005;23:355-364.  
Sasaki K, Inoue M, Shibata H, Ueda Y, Muramatsu S, Okada T, Hasegawa M, Ozawa K, Hanazono Y. Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved. Gene Ther 2005 Feb;12(3):203-210.  
Sasaki K, Nagao Y, Kitano Y, Hasegawa H, Shibata H, Takatoku M, Hayashi S, Ozawa K, Hanazono Y. Hematopoietic microchimerism in sheep after in utero transplantation of cultured cynomolgus embryonic stem cells. Transplantation 2005 Jan 15;79(1):32-37.   
2004 Ueda K, Hanazono Y, Shibata H, Ageyama N, Ueda Y, Ogata S, Tabata T, Nagashima T, Takatoku M, Kume A, Ikehara S, Taniwaki M, Terao K, Hasegawa M, Ozawa K. High-level in vivo gene marking after gene-modified autologous hematopoietic stem cell transplantation without marrow conditioning in nonhuman primates. Mol Ther 2004 Sep;10(3):469-477.  
Nagashima T, Ueda Y, Hanazono Y, Kume A, Shibata H, Ageyama N, Terao K, Ozawa K, Hasegawa M. In vivo expansion of gene-modified hematopoietic cells by a novel selective amplifier gene utilizing the erythropoietin receptor as a molecular switch. J Gene Med 2004 Jan;6(1):22-31.  
 2003 Itoh A, Okada T, Mizuguchi H, Hayakawa T, Mizukami H, Kume A, Takatoku M, Komatsu N, Hanazono Y, Ozawa K. A soluble CAR-SCF fusion protein improves adenoviral vector-mediated gene transfer to c-Kit-positive hematopoietic cells. J Gene Med 2003 Nov;5(11):929-40.  
Asano T, Ageyama N, Takeuchi K, Momoeda M, Kitano Y, Sasaki K, Ueda Y, Suzuki Y, Kondo Y, Torii R, Hasegawa M, Ookawara S, Harii K, Terao K, Ozawa K, Hanazono Y. Engraftment and tumor formation after allogeneic in utero transplantation of primate embryonic stem cells. Transplantation 2003 Oct 15;76(7):1061-1067.  
Shibata H, Hanazono Y, Ageyama N, Nagashima T, Ueda Y, Hasegawa M, Ozawa K, Yoshikawa Y, Terao K. Collection and analysis of hematopoietic progenitor cells from cynomolgus macaques (Macaca fascicularis): Assessment of cross-reacting monoclonal antibodies. Am J Primatol 2003 Sep;61(1):3-12.  
Nagashima T, Ueda Y, Hanazono Y, Kume A, Shibata H, Ageyama N, Terao K, Ozawa K, Hasegawa M. In vivo expansion of gene-modified hematopoietic cells by a novel selective amplifier gene utilizing the erythropoietin receptor as a molecular switch. Biochem Biophys Res Commun 2003 Mar 28;303(1):170-6.  
Hanazono Y, Asano T, Ueda Y, Ozawa K. Genetic manipulation of primate embryonic and hematopoietic stem cells with simian lentivirus vectors. Trends Cardiovasc Med 2003 Apr;13(3):106-110.  
2002 Ageyama N, Hanazono Y, Shibata H, Ohto K, Ono F, Nagashima T, Ueda Y, Donahue RE, Hasegawa M, Ozawa K, Yoshikawa Y, Terao K. Safe and efficient methods of autologous hematopoietic stem cell transplantation for biomedical research in cynomolgus monkeys. Comp Med. 2002 (Oct);52:445-451.  
Hanazono Y, Terao K, Shibata H, Nagashima T, Ageyama N, Asano T, Ueda Y, Kato I, Kume A, Hasegawa M, Ozawa K. Introduction of the GFP gene into hematopoietic stem cells results in prolonged discrepancy of in vivo transduction levels between bone marrow progenitors and peripheral blood cells in nonhuman primates. J Gene Med 2002 (Sep);4:470-477.  
Asano T, Hanazono Y, Ueda Y, Muramatsu S, Kume A, Suemori H, Suzuki Y, Kondo Y, Harii K, Hasegawa M, Nakatsuji N, Ozawa K. Highly efficient gene transfer into primate embryonic stem cells with a simian lentivirus vector. Mol Ther 2002 (Aug);6:162-168.  
Hanazono Y, Nagashima T, Takatoku M, Shibata H, Ageyama N, Asano T, Ueda Y, Dunbar CE, Kume A, Terao K, Hasegawa M, Ozawa K. In vivo selective expansion of gene-modified hematopoietic cells in a nonhuman primate model. Gene Ther 2002 (Aug);9:1055-1064.  
 2001 Hanazono Y, Terao K, Ozawa K. Gene transfer into nonhuman primate hematopoietic stem cells: implications for gene therapy. Stem Cells 2001;19:12-23.