{"id":1136,"date":"2020-09-29T17:45:22","date_gmt":"2020-09-29T08:45:22","guid":{"rendered":"https:\/\/www.jichi.ac.jp\/alle\/cms\/?p=1136"},"modified":"2020-09-29T17:45:22","modified_gmt":"2020-09-29T08:45:22","slug":"%e7%a7%81%e7%9a%84%e5%85%8d%e7%96%ab%e5%ad%a6%e3%81%93%e3%81%a8%e3%81%af%e3%81%98%e3%82%81-22%e3%80%80aire%e7%a0%94%e7%a9%b62%e3%80%80-rnase-protection-assayrpa","status":"publish","type":"post","link":"https:\/\/www.jichi.ac.jp\/alle\/%e6%95%99%e6%8e%88%e3%81%b6%e3%82%8d%e3%81%90%ef%bc%88%e8%b6%85%e4%b8%8d%e5%ae%9a%e6%9c%9f%e6%8e%b2%e8%bc%89%ef%bc%89\/%e7%a7%81%e7%9a%84%e5%85%8d%e7%96%ab%e5%ad%a6%e3%81%93%e3%81%a8%e3%81%af%e3%81%98%e3%82%81-22%e3%80%80aire%e7%a0%94%e7%a9%b62%e3%80%80-rnase-protection-assayrpa\/","title":{"rendered":"\u79c1\u7684\u514d\u75ab\u5b66\u3053\u3068\u306f\u3058\u3081 (22)\u3000Aire\u7814\u7a76(2)\u3000 RNase protection assay(RPA)"},"content":{"rendered":"<p><span style=\"color: #000080; font-size: 12pt;\">RNase protection assay(RPA)\u3068\u3044\u3046\u306e\u306fmRNA\u306e\u5b9a\u91cf\u306b\u4f7f\u3046\u624b\u6cd5\u3067\u3001\u305d\u306e\u70b9\u3067\u306fNorthern blotting\u306b\u4f3c\u3066\u3044\u307e\u3059\u3002\u305f\u3060\u3057\u5f8c\u8005\u3067\u306f\u3001\u30d0\u30f3\u30c9\u306e\u4f4d\u7f6e\uff08\u6cf3\u52d5\u8ddd\u96e2\uff09\u306fmRNA\u306e\u30b5\u30a4\u30ba\u3092\u793a\u3059\u306e\u306b\u5bfe\u3057\u3066\u3001RPA\u3067\u306f\u4e88\u3081\u8a2d\u8a08\u3057\u305f\u30d7\u30ed\u30fc\u30d6\u306e\u9577\u3055\u3067\u6c7a\u307e\u308a\u307e\u3059\u3002\u3053\u306e\u6027\u8cea\u3092\u5229\u7528\u3057\u3066\u3001\u4e00\u5ea6\u306e\u96fb\u6c17\u6cf3\u52d5\u3067\u8907\u6570\u7a2e\u985e\u306e\u5206\u5b50\u306emRNA\u3092\u5b9a\u91cf\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3059\u3002<\/span><\/p>\n<p><span style=\"color: #000080; font-size: 12pt;\">\u305d\u306e\u3088\u3046\u306a\u30ad\u30c3\u30c8\u304c\u3042\u308b\u306e\u3092\u77e5\u3063\u3066\u3001\u3084\u3063\u3066\u307f\u305f\u3044\u3068\u601d\u3063\u3066\u3044\u305f\u306e\u3067\u3057\u305f\u3002\u305f\u3060mRNA\u306f\u4e0d\u5b89\u5b9a\u306a\u5206\u89e3\u3055\u308c\u3084\u3059\u3044\u5206\u5b50\u3067\u3059\u3057\u3001\u7d50\u69cb\uff08\u79c1\u306b\u3068\u3063\u3066\u306f\uff09\u96e3\u5ea6\u306e\u9ad8\u3044\u5b9f\u9a13\u3067\u3057\u305f\u3002\u5468\u56f2\u306bRPA\u3092\u3084\u3063\u3066\u3044\u308b\u4eba\u3082\u3044\u306a\u304b\u3063\u305f\u306e\u3067\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u306b\u6642\u9593\u304c\u304b\u304b\u308a\u307e\u3057\u305f\u3002\u305d\u308c\u3067\u3082\u3069\u3093\u306a\u7d50\u679c\u304c\u51fa\u308b\u306e\u304b\u3001\u30ef\u30af\u30ef\u30af\u3057\u306a\u304c\u3089\u3084\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u7d50\u679c\u3092\u898b\u308b\u3068\u3001Aire\u3092\u5f37\u5236\u767a\u73fe\u3057\u305f\u5358\u7403\u7cfb\u306e\u7d30\u80de\u682a\u3067\u306f\u3001IL-1 receptor antagonist (IL-1Ra)\u3068\u3044\u3046\u3001\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3IL-1\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u963b\u5bb3\u3059\u308b\u5206\u5b50\u306e\u767a\u73fe\u91cf\u304c\u4e0b\u304c\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f<a style=\"color: #000080;\" href=\"https:\/\/www.jichi.ac.jp\/alle\/cms\/wp-content\/uploads\/dd42f520d03e524f1dc308e573e1dab1.jpg\" rel=\"lightbox[1136]\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-1137 alignleft\" src=\"https:\/\/www.jichi.ac.jp\/alle\/cms\/wp-content\/uploads\/dd42f520d03e524f1dc308e573e1dab1.jpg\" alt=\"\" width=\"282\" height=\"319\" srcset=\"https:\/\/www.jichi.ac.jp\/alle\/cms\/wp-content\/uploads\/dd42f520d03e524f1dc308e573e1dab1.jpg 506w, https:\/\/www.jichi.ac.jp\/alle\/cms\/wp-content\/uploads\/dd42f520d03e524f1dc308e573e1dab1-265x300.jpg 265w\" sizes=\"auto, (max-width: 282px) 100vw, 282px\" \/><\/a>\uff08\u4e00\u4f8b\u3092\u56f3\u306b\u793a\u3057\u307e\u3059\u3002\u307e\u3063\u3059\u3050\u306b\u6cf3\u52d5\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u304c\u30fb\u30fb\u30fb\u3002\u5de6\u5074\u306fIL-12p35, IL-10, IL-1\u03b1, IL-1\u03b2, IL-1Ra, IL-18, IL-6, IFN-\u03b3, MIF\u3092\u3001\u53f3\u5074\u306f Ltn, RANTES, Eotaxin, MIP-1\u03b2, MIP-1\u03b1, MIP-2, IP-10, MCP-1, TCA-3\u3092\u4e00\u5ea6\u306b\u6e2c\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002L32\u3068GAPDH\u306f\u305f\u3044\u3066\u3044\u306e\u5834\u5408\u3067\u8a98\u5c0e\u3055\u308c\u308b\u5bfe\u7167\u5206\u5b50\u3067\u3059\u304c\u3001\u4ed6\u306e\u5206\u5b50\u306f\u5168\u7136\u6e2c\u5b9a\u51fa\u6765\u306a\u3044\u3082\u306e\u3082\u3042\u308a\u307e\u3059\u3057\u3001\u6e2c\u5b9a\u51fa\u6765\u308b\u3082\u306e\u3067\u3082\u3001\u7121\u523a\u6fc0\u3067\u306f\u5168\u7136\u767a\u73fe\u3057\u3066\u304a\u3089\u305a\u3001\u30ea\u30dd\u30dd\u30ea\u30b5\u30c3\u30ab\u30e9\u30a4\u30c9(LPS, \u83cc\u4f53\u6210\u5206)\u3067\u5f37\u529b\u306b\u523a\u6fc0\u3057\u3066\u3088\u3046\u3084\u304f\u8a98\u5c0e\u3055\u308c\u308b\u3082\u306e\u304c\u591a\u3044\u3088\u3046\u3067\u3059\u3002\uff09\u3002<\/span><br \/>\n<span style=\"color: #000080; font-size: 12pt;\">\u6b63\u306e\u8ee2\u5199\u56e0\u5b50\u3068\u601d\u308f\u308c\u3066\u3044\u305f\u306e\u306b\u767a\u73fe\u91cf\u304c\u4e0b\u304c\u308b\u3068\u3044\u3046\u306e\u3082\u4e0d\u601d\u8b70\u306a\u8a71\u3067\u3059\u304c\u3001IRF\u30d5\u30a1\u30df\u30ea\u30fc\u306eIRF-2\u306e\u3088\u3046\u306b\u3001\u8ca0\u306e\u8ee2\u5199\u56e0\u5b50\u306e\u5074\u9762\u304c\u3042\u308b\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u4e00\u7b4b\u7e04\u3067\u306f\u3044\u304b\u306a\u3044\u3082\u306e\u3067\u3059\u3002\u672c\u5f53\u306f\u8ee2\u5199\u56e0\u5b50\u3067\u306f\u306a\u304f\u3066\u3001\u8ee2\u5199\uff62\u8abf\u7bc0\uff63\u56e0\u5b50\u3068\u3044\u3046\u53ef\u80fd\u6027\u3082\u3042\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/span><\/p>\n<p style=\"text-align: right;\"><span style=\"color: #808080; font-size: 12pt;\"><strong>\u4f50\u85e4\u3000\u6d69\u4e8c\u90ce<\/strong><\/span><\/p>\n<p style=\"text-align: right;\"><span style=\"font-size: 11pt;\"><a href=\"https:\/\/www.jichi.ac.jp\/alle\/cms\/%e6%95%99%e6%8e%88%e3%81%b6%e3%82%8d%e3%81%90%ef%bc%88%e8%b6%85%e4%b8%8d%e5%ae%9a%e6%9c%9f%e6%8e%b2%e8%bc%89%ef%bc%89\/%e7%a7%81%e7%9a%84%e5%85%8d%e7%96%ab%e5%ad%a6%e3%81%93%e3%81%a8%e3%81%af%e3%81%98%e3%82%81-21%e3%80%80aire%e7%a0%94%e7%a9%b61%e3%80%80\"><span style=\"color: #000080;\">\u79c1\u7684\u514d\u75ab\u5b66\u3053\u3068\u306f\u3058\u3081 (21)<\/span><\/a>\u00a0\u00a0<span style=\"color: #0000ff;\"><strong><em>\u2190 Prev\u3000\u3000\u3000\u3000\u3000<\/em><\/strong><\/span><\/span><strong><em><span style=\"font-size: 11pt;\"><span style=\"color: #0000ff;\">Next<\/span><\/span><\/em><\/strong><span style=\"font-size: 11pt;\"><span style=\"color: #0000ff;\"> \u2192<\/span><\/span><a href=\"https:\/\/www.jichi.ac.jp\/alle\/cms\/%e6%95%99%e6%8e%88%e3%81%b6%e3%82%8d%e3%81%90%ef%bc%88%e8%b6%85%e4%b8%8d%e5%ae%9a%e6%9c%9f%e6%8e%b2%e8%bc%89%ef%bc%89\/%e7%a7%81%e7%9a%84%e5%85%8d%e7%96%ab%e5%ad%a6%e3%81%93%e3%81%a8%e3%81%af%e3%81%98%e3%82%81-23-aire%e7%a0%94%e7%a9%b63-%e6%9c%ac%e5%bd%93%e3%81%af%e3%82%84%e3%82%8a%e3%81%9f%e3%81%8b%e3%81%a3\"><span style=\"font-size: 11pt;\">\u79c1\u7684\u514d\u75ab\u5b66<\/span><span style=\"font-size: 11pt;\">\u3053\u3068\u306f\u3058\u3081<\/span><span style=\"font-size: 11pt;\"> (23)<\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"RNase protection assay(RPA)\u3068\u3044\u3046\u306e\u306fmRNA\u306e\u5b9a\u91cf\u306b\u4f7f\u3046\u624b\u6cd5\u3067\u3001\u305d\u306e\u70b9\u3067\u306fNorthern blotting\u306b\u4f3c\u3066\u3044\u307e\u3059\u3002\u305f\u3060\u3057\u5f8c\u8005\u3067\u306f\u3001\u30d0\u30f3\u30c9\u306e\u4f4d\u7f6e\uff08\u6cf3\u52d5\u8ddd\u96e2\uff09\u306fmRNA\u306e\u30b5\u30a4\u30ba\u3092\u793a\u3059\u306e\u306b\u5bfe &#8230;","protected":false},"author":1,"featured_media":1137,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-1136","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-8"],"_links":{"self":[{"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/posts\/1136","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/comments?post=1136"}],"version-history":[{"count":0,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/posts\/1136\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/media\/1137"}],"wp:attachment":[{"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/media?parent=1136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/categories?post=1136"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/alle\/wp-json\/wp\/v2\/tags?post=1136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}