sGRAPHIC
Secretory GRAPHIC (sGRAPHIC) is a genetically encoded tool to fluorescently label neighboring cells that putatively interact with cells of interest via secretory factors (Nat Commun 2023; 14: 8031). The fundamental design of sGRAPHIC is inspired with GRAPHIC system (iScience 2019, 15, 28). Similar to GRAHIC, sGRAPHIC labelling is based on split GFP technology: functional GFP is efficiently reconstituted when cells expressing GPI-anchored N-terminal GFP and cells expressing secretory C-terminal GFP are in close proximity.
The plasmids listed below for lentivirus production are available in RIKEN BRC.
CSⅡ/CMV-nGRAPHIC-T2A-H2B-mCherry [map] [RIKEN BRC] (iScience 2019, Nat Commun 2023)
CSⅡ/CMV-ss-c-sfGFP-LZB-GPI-T2A-H2B-Azurite [map] [RIKEN BRC](Nat Commun 2023)
CSⅣ/TRE-scGRAPHIC-T2A-H2B-Azurite-CMV-rtTA [map] [RIKEN BRC](unpublished)
CSⅡ/CMV-nGRAPHIC-P2A-mCherry [map] [RIKEN BRC] (unpublished)
CSⅡ/CMV-scGRAPHIC-P2A-mTagBFP2 [map] (unpublished)
CSⅣ/TRE-scGRAPHIC-T2A-mTagBFP2-CMV-rtTA [map] (unpublished)