{"id":769,"date":"2024-10-03T15:17:24","date_gmt":"2024-10-03T06:17:24","guid":{"rendered":"https:\/\/www.jichi.ac.jp\/bioconvergence\/?p=769"},"modified":"2025-12-07T21:27:40","modified_gmt":"2025-12-07T12:27:40","slug":"sgraphic-en","status":"publish","type":"post","link":"https:\/\/www.jichi.ac.jp\/bioconvergence\/en\/sgraphic-en\/","title":{"rendered":"sGRAPHIC"},"content":{"rendered":"<p>Secretory GRAPHIC (sGRAPHIC) is a genetically encoded tool to fluorescently label neighboring cells that putatively interact with cells of interest via secretory factors (<a href=\"https:\/\/www.nature.com\/articles\/s41467-023-43855-2\">Nat Commun 2023; 14: 8031<\/a>). The fundamental design of sGRAPHIC is inspired with GRAPHIC system (<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2589004219301105\">iScience 2019, 15, 28<\/a>). Similar to GRAHIC, sGRAPHIC labelling is based on split GFP technology: functional GFP is efficiently reconstituted when cells expressing GPI-anchored N-terminal GFP and cells expressing secretory C-terminal GFP are in close proximity.<\/p>\n<p>The plasmids listed below for lentivirus production are available in RIKEN BRC.<\/p>\n<p>&nbsp;<\/p>\n<p>CS\u2161\/CMV-nGRAPHIC-T2A-H2B-mCherry [<a href=\"https:\/\/benchling.com\/s\/seq-nQlKmGxJMziwHdcND9Iv?m=slm-M2eLr3KTJRgDo1gSw6BH\">map<\/a>] [<a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB15\/RDB15870.html\">RIKEN BRC<\/a>] (iScience 2019, Nat Commun 2023)<\/p>\n<p>CS\u2161\/CMV-ss-c-sfGFP-LZB-GPI-T2A-H2B-Azurite [<a href=\"https:\/\/benchling.com\/s\/seq-ZsnryffmZWARUc9CuDcm?m=slm-i3ugz2FrFgsd33Nl9L9T\">map<\/a>] [<a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB20\/RDB20427.html\">RIKEN BRC<\/a>](Nat Commun 2023)<\/p>\n<p>CS\u2163\/TRE-scGRAPHIC-T2A-H2B-Azurite-CMV-rtTA [<a href=\"https:\/\/benchling.com\/s\/seq-AdE6dHkl1tQN9VPzq7SA?m=slm-Mt0tKeyRXt0KvyUzR0pS\">map<\/a>] [<a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB20\/RDB20428.html\">RIKEN BRC<\/a>](unpublished)<\/p>\n<p>CS\u2161\/CMV-nGRAPHIC-P2A-mCherry [<a href=\"https:\/\/benchling.com\/s\/seq-GjMy4Z0cHJd9gXpSOD9D?m=slm-iBsbu3EwVa0cGMuH9hc2\">map<\/a>] [<a href=\"https:\/\/dnaconda.riken.jp\/search\/RDB_clone\/RDB20\/RDB20429.html\">RIKEN BRC<\/a>] (unpublished)<\/p>\n<p>CS\u2161\/CMV-scGRAPHIC-P2A-mTagBFP2 [<a href=\"https:\/\/benchling.com\/s\/seq-IJ64GfWAsTitbNzRfoN5?m=slm-Xgel4wZf4FGyC6mtHFvd\">map<\/a>] (unpublished)<\/p>\n<p>CS\u2163\/TRE-scGRAPHIC-T2A-mTagBFP2-CMV-rtTA [<a href=\"https:\/\/benchling.com\/s\/seq-qmnRBTnXGdlLETRqnO6X?m=slm-1Y7VM6rcLY6hq6ZkiegA\">map<\/a>] (unpublished)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Secretory GRAPHIC (sGRAPHIC) is a genetically encoded tool to fluorescently label neighboring cells that putat [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":1313,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[97],"tags":[],"class_list":["post-769","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-resource-en"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/posts\/769","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/comments?post=769"}],"version-history":[{"count":12,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/posts\/769\/revisions"}],"predecessor-version":[{"id":1324,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/posts\/769\/revisions\/1324"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/media\/1313"}],"wp:attachment":[{"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/media?parent=769"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/categories?post=769"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.jichi.ac.jp\/bioconvergence\/wp-json\/wp\/v2\/tags?post=769"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}