研究成果 - 生物物理学部門

研究成果PUBLICATIONS

発表論文

2023
1. Development of serial X-ray fluorescence holography for radiation-sensitive protein crystals.
  Ang AKR, Umena Y, Sato-Tomita A, Shibayama N, Happo N, Marumi R, Yamamoto Y, Kimura K, Kawamura N, Takano Y, Matsushita T, Sasaki YC, Sheni J-R, Hayashi K
  Journal of Synchrotron Radiation 30, 368-378, 2023年3月
  https://doi.org/10.1107/S1600577522011833
2. Incoherent Neutron Scattering and Terahertz Time-Domain Spectroscopy on Protein and Hydration Water.
  Nakagawa H, Yamamoto N
  Life 13, 2 318-318, 2023年1月
  https://doi.org/10.3390/life13020318
2022
1. Tracking the Structural Development of Amyloid Precursors in the Insulin B Chain and the Inhibition Effect by Fibrinogen.
  Yamamoto N, Inoue R, Makino Y, Sekiguchi H, Shibayama N, Naito A, Sugiyama M, Chatani E
  The Journal of Physical Chemistry B 126, 10797-10812, 2022年12月
  https://doi.org/10.1021/acs.jpcb.2c05136
2. X-ray fluorescence holography of biological metal sites: Application to myoglobin.
  Sato-Tomita A, Ang AKR, Kimura K, Marumi R, Happo N, Matsushita T, Park S-Y, Shibayama N, Sasaki YC, Hayashi K
  Biochemical and Biophysical Research Communications 635, 277-282, 2022年 10月
  https://doi.org/10.1016/j.bbrc.2022.10.003
3. Protein large-scale motions revealed by quantum beams: A new era in understanding protein dynamics.
  Yamamoto N, Inoue R, Kurisaki I, Matsuo T, Hishikawa Y, Zhao W, Sekiguchi H
  Biophysics and Physicobiology 19, e190035, 2022 年9月
  https://doi.org/10.2142/biophysico.bppb-v19.0035
4. Heme-bound tyrosine vibrations in hemoglobin M: Resonance Raman, crystallography, and DFT calculation.
  Nagatomo S, Shoji M, Terada T, Nakatani K, Shigeta Y, Hirota S, Yanagisawa S, Kubo M, Kitagawa T, Nagai M, Ohki M, Park S-Y, Shibayama N
  Biophysical journal 121, 2767-2780, 2022年 7月
  https://doi.org/10.1016/j.bpj.2022.06.012
5. Multistep growth of amyloid intermediates and its inhibition toward exploring therapeutic way: A case study using insulin B chain and fibrinogen.
  Yamamoto N, Chatani E
  Biophysics and Physicobiology 19, e190017, 2022 年6月
  https://doi.org/10.2142/biophysico.bppb-v19.0017
6. Crystalline-state chemiluminescence reactions of two-fluorophore-linked adamantylideneadamantane 1,2-dioxetane isomers accompanied by solid-to-solid phase transitions.
  Matsuhashi C, Oyama H, Uekusa H, Sato-Tomita A, Ichiyanagi K, Maki S, Hirano T
  CrystEngComm 24, 3309-3494, 2022年 5月
  https://doi.org/10.1039/D2CE00266C
7. Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system.
  Yamamoto N, Kikuchi J, Furukawa Y, Shibayama N
  PLOS ONE 17, e0261699, 2022年5月
  https://doi.org/10.1371/journal.pone.0261699
2021
1. Protein hydration and its freezing phenomena: Toward the application for cell freezing and frozen food storage.
  Yamamoto N, Nakanishi M, Rajan R, Nakagawa H
  Biophysics and Physicobiology 18, 284-288, 2021年11月
  https://doi.org/10.2142/biophysico.bppb-v18.034
2. Laboratory diffracted X-ray blinking to monitor picometer motions of protein molecules and application to crystalline materials.
  Arai T, Inamasu R, Yamaguchi H, Sasaki D, Sato-Tomita A, Sekiguchi H, Mio K, Tsuda S, Kuramochi M, Sasaki YC
  Structural Dynamics 8, 044302, 2021年7月
  https://aca.scitation.org/doi/10.1063/4.0000112
3. アミロイド線維前駆中間体の観測とそれを標的とした線維形成阻害―インスリン由来モデルペプチドとフィブリノーゲンを用いた検証―
  山本直樹, 茶谷絵理
  生物物理 61(4), 236-239, 2021年7月
  https://doi.org/10.2142/biophys.61.236
4. Elucidation of local structure deformation in κ−(BEDT−TTF)₂Cu[N(CN)₂]Br by X-ray fluorescence holography.
  Ang　AKR, Marumi R, Sato-Tomita A, Kimura K, Happo N, Akagi K, Sasaki T, Hayashi K
  Physical Review B 103, 214106, 2021年5月
  https://doi.org/10.1103/PhysRevB.103.214106
5. Vapochromic behaviour of a nickel(II)-quinonoid complex with dimensional changes between 1D and higher.
  Yano R, Yoshida M, Tsunenari T, Sato-Tomita A, Nozawa S, Iida Y, Matsunaga N, Kobayashi A, Kato M
  Dalton Transactions 50, 8696-8703, 2021年4月
  https://doi.org/10.1039/D1DT00269D
6. Freezable and unfreezable hydration water: Distinct contributions to protein dynamics revealed by neutron scattering.
  Yamamoto N, Kofu M, Nakajima K, Nakagawa H, Shibayama N
  The Journal of Physical Chemistry Letters 12(8), 2172-2176, 2021年2月
  https://doi.org/10.1021/acs.jpclett.0c03786
7. 放射光X 線を用いた時間分解結晶構造解析法
  佐藤文菜, 梅名泰史, 一柳光平, 深谷亮, 引田理英, 松垣直宏, 務台俊樹
  日本結晶学会誌 63, 24-30, 2021年1月
  https://doi.org/10.5940/jcrsj.63.24
2020
1. Multistep changes in amyloid structure induced by cross-seeding on a rugged energy landscape.
  Yuzu K, Yamamoto N, Noji M, So M, Goto Y, Iwasaki T, Tsubaki M, Chatani E
  Biophysical Journal 120(2), 284-295, 2020年12月
  https://doi.org/10.1016/j.bpj.2020.12.005
2. Iodine staining as a useful probe for distinguishing insulin amyloid polymorphs.
  Hiramatsu T, Yamamoto N, Ha S, Masuda Y, Yasuda M, Ishigaki M, Yuzu K, Ozaki Y, Chatani E
  Scientific Reports 10(1), 16741, 2020年12月
  https://doi.org/10.1038/s41598-020-73460-y
3. 放射光X線をプローブとしたソフトクリスタル構造解析～ポンプ-プローブ結晶構造解析と差フーリエ電子マップ～
  佐藤文菜
  化学と工業 73巻8月号, 627-628, 2020年8月
  https://www.chemistry.or.jp/journal/chemical-industry/vol73-no8.html
4. Isomeric difference in the crystalline-state chemiluminescence property of an adamantylideneadamantane 1,2-dioxetane with a phthahlimide chromophore.
  Matsuhashi C, Ueno T, Uekusa H, Sato-Tomita A, Ichiyanagi K, Maki S, Hirano T
  Chemical communications 56, 3369-3372, 2020年2月
  https://doi.org/10.1039/C9CC10012A
5. Direct observation of ligand migration within human hemoglobin at work.
  Shibayama N, Sato-Tomita A, Ohki M, Ichiyanagi K, Park S-Y
  Proceedings of the National Academy of Sciences of the United States of America 117, 4741-4748, 2020年2月
  https://doi.org/10.1073/pnas.1913663117
6. Development of shock-dynamics study with synchrotron-based time-resolved X-ray diffraction using an Nd:glass laser system.
  Takagi S, Ichiyanagi K, Kyono A, Nozawa S, Kawai N, Fukaya R, Funamori N, Adachi S
  Journal of Synchrotron Radiation 27, 371-377, 2020年2月
  https://doi.org/10.1107/S1600577519016084
7. Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family.
  Yun J-H, Ohki M, Park J-H, Ishimoto N, Sato-Tomita A, Lee W, Jin Z, Tame JRH, Shibayama N, Park S-Y, Lee W
  Science Advances 6, eaay2042, 2020年2月
  https://doi.org/10.1126/sciadv.aay2042
8. X-ray fluorescence holography for soft matter.
  Ang AKR, Sato-Tomita A, Shibayama N, Umena Y, Happo N, Marumi R, Kimura K, Matsushita T, Akagi K, Sasaki T, Sasaki YC, Hayashi K
  Japanese Journal of Applied Physics 59, 010505, 2020年1月
  https://doi.org/10.7567/1347-4065/ab5d55
2019
1. Fast and stable vapochromic response induced through nanocrystal formation of a luminescent platinum(II) complex on periodic mesoporous organosilica.
  Matsukawa H, Yoshida M, Tsunenari T, Nozawa S, Sato-Tomita A, Maegawa Y, Inagaki S, Kobayashi A, Kato M
  Scientific Reports 9, 15151, 2019年10月
  https://doi.org/10.1038/s41598-019-51615-w
2. Broadband dielectric spectroscopy from sub GHz to THz of hydrated lipid bilayer of DMPC.
  Kadomura Y, Yamamoto N, Tominaga K
  European Physical Journal E 42, 139, 2019年10月
  https://link.springer.com/article/10.1140/epje/i2019-11901-1
3. Exergonic Intramolecular Singlet Fission of an Adamantanelinked Tetracene Dyad via Twin Quintet Multiexcitons.
  Matsui Y, Kawaoka S, Nagashima H, Nakagawa T, Okamura N, Ogaki T, Ohta E, Akimoto S, Sato-Tomita A, Yagi S, Kobori Y, Ikeda H
  The Journal of Physical Chemistry, 123, 18813-18823, 2019年7月
  https://pubs.acs.org/doi/pdf/10.1021/acs.jpcc.9b04503
4. Shape Rememorization of an Organosuperelastic Crystal via Superelasticity-Ferroelasticity Interconversion.
  Sakamoto S, Sasaki T, Sato-Tomita A, Takamizawa S
  Angewandte Chemie International Edition 58, 13722-13726, 2019年7月
  https://doi.org/10.1002/anie.201905769
5. Microstructural deformation process of shock-compressed polycrystalline aluminum.
  Ichiyanagi K, Takagi S, Kawai N, Fukaya R, Nozawa S, Nakamura KG, Liss K-D, Kimura M, Adachi S
  Scientific Reports 9, 7604, 2019年5月
  https://doi.org/10.1038/s41598-019-43876-2
6. Structural Insights into the Inhibition of Amyloid Fibril Formation by Fibrinogen via Interaction with Prefibrillar Intermediates.
  Yamamoto N, Akai T, Inoue R, Sigiyama M, Tamura A, Chatani E
  Biochemistry 58, 2769-2781, 2019年 3月
  https://pubs.acs.org/doi/10.1021/acs.biochem.9b00439
7. Allosteric transitions in hemoglobin revisited.
  Shibayama N
  Biochimica et Biophysica Acta - General Subjects 1864, 129335, 2020年 (2019年3月 Accepted)
  https://doi.org/10.1016/j.bbagen.2019.03.021
2018
1. Direct Imaging of Valence-Sensitive X-Ray Fluorescence Holograms of Fe3O4.
  Ang AKR, Matsushita T, Hashimoto Y, Happo N, Yamamoto Y, Mizuguchi M, Sato-Tomita A, Shibayama N, Sasaki YC, Kimura K, Taguchi M, Daimon H, Hayashi K
  Physica Status Solidi B 255, 1800100 2018年6月
  https://www.onlinelibrary.wiley.com/doi/10.1002/pssb.201800100
2. Progressing of 3D Protein Structure and Dynamics Measurements.
  Sato-Tomita A, Sekiguchi H, Sasaki YC
  Journal of the Physical Society of Japan 87, 061015 2018年4月
  https://doi.org/10.7566/JPSJ.87.061015
3. Principle and Reconstruction Algorithm for Atomic-Resolution Holography.
  Matsushita T, Muro T, Matsui M, Happo N, Hosokawa S, Ohoyama K, Sato-Tomita A, Sasaki YC, Hayashi K
  Journal of the Physical Society of Japan 87, 061002 2018年4月
  https://doi.org/10.7566/JPSJ.87.061002
4. Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages.
  Karasawa T, Kawashima A, Usui-Kawanishi F, Watanabe S, Kimura H, Kamata R, Shirasuna K, Koyama Y, Sato-Tomita A, Matsuzaka T, Tomoda H, Park SY, Shibayama N, Shimano H, Kasahara T, Takahashi M
  Arteriosclerosis, Thrombosis, and Vascular Biology 38, 744-756, 2018年2月
  https://www.ahajournals.org/doi/10.1161/ATVBAHA.117.310581
2017
1. Direct observation of conformational population shifts in crystalline human hemoglobin.
  Shibayama N, Ohki M, Tame JRH, Park SY
  The Journal of Biological Chemistry 292, 18258-18269 2017年9月
  http://www.jbc.org/content/292/44/18258.short
2. Size and Shape Controlled Crystallization of Hemoglobin for Advanced Crystallography.
  Sato-Tomita A, Shibayama N
  Crystals 7(9), 282 2017年9月
  http://www.mdpi.com/2073-4352/7/9/282
3. Ligation-Dependent Picosecond Dynamics in Human Hemoglobin As Revealed by Quasielastic Neutron Scattering.
  Fujiwara S, Chatake T, Matsuo T, Kono F, Tominaga T, Shibata K, Sato-Tomita A, Shibayama N
  The journal of physical chemistry B 121(34) 8069-8077 2017年8月
  http://pubs.acs.org/doi/abs/10.1021/acs.jpcb.7b05182
4. Molecular mechanism of photoactivation of a light-regulated adenylate cyclase.
  Ohki M, Sato-Tomita A, Matsunaga S, Iseki M, Tame JRH, Shibayama N, Park SY
  Proceedings of the National Academy of Sciences of the United States of America 114(32) 8562-8567 2017年8月
  http://www.pnas.org/content/114/32/8562
5. Protein crystallography beamline BL2S1 at the Aichi synchrotron.
  Watanabe N, Nagae T, Yamada Y, Tomita A, Matsugaki N, Tabuchi M
  J Synchrotron Radiation 24(Pt 1) 338-43 2017年1月
  https://doi.org/10.1107/S1600577516018579
2016
1. Development of an X-ray fluorescence holographic measurement system for protein crystals.
  Sato-Tomita A, Shibayama N, Happo N, Kimura K, Okabe T, Matsushita T, Park SY, Sasaki YC, Hayashi K
  The Review of scientific instruments 87(6) 063707 2016年6月
  http://dx.doi.org/10.1063/1.4953453
2. Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium.
  Ohki M, Sugiyama K, Kawai F, Tanaka H, Nihei Y, Unzai S, Takebe M, Matsunaga S, Adachi S, Shibayama N, Zhou ZW, Koyama R, Ikegaya Y, Takahashi T, Tame JRH, Iseki M, Park SY
  Proceedings of the National Academy of Sciences of the United States of America 113(24) 6659-6664 2016年6月
  http://www.pnas.org/content/113/24/6659
2015
1. Loop L1 governs the DNA-binding specificity and order for RecA-catalyzed reactions in homologous recombination and DNA repair.
  Shinohara T, Ikawa S, Iwasaki W, Hiraki T, Hikima T, Mikawa T, Arai N, Kamiya N, Shibata T
  Nucleic acids research 43(2) 973-986 2015年01月
  http://dx.doi.org/10.1093/nar/gku1364
2. Cooling dynamics of self-assembled monolayer coating for integrated gold nanocrystals on a glass substrate.
  
  Ichiyanagi K, Sekiguchi H, Sato T, Nozawa S, Tomita A, Hoshino M, Adachi S, Sasaki YC
  
  Journal of synchrotron radiation 22(Pt 1) 29-33 2015年01月
  http://dx.doi.org/10.1107/S1600577514019730
2014
1. ポンプ－プローブ法を用いた生体分子の構造ダイナミクス測定
  
  富田文菜, 野澤俊介, 足立伸一, 佐藤篤志, 柴山修哉
  
  日本結晶学会誌 56(4) 253-258 2014年08月
  http://dx.doi.org/10.5940/jcrsj.56.253
2. Delineation of Solution Burst-Phase Protein Folding Events by Encapsulating the Proteins in Silica Gels.
  Okabe T, Tsukamoto S, Fujiwara K, Shibayama N, Ikeguchi M
  Biochemistry. 53(23) 3858-66 2014年6月
  http://pubs.acs.org/doi/abs/10.1021/bi5003647
3. Capturing the hemoglobin allosteric transition in a single crystal form.
  Shibayama N, Sugiyama K, Tame JR, Park SY
  Journal of the American Chemical Society 136 5097-5105 2014年04月
  http://dx.doi.org/10.1021/ja500380e
2013
1. Intersubunit communication via changes in hemoglobin quaternary structures revealed by time-resolved resonance Raman spectroscopy: direct observation of the Perutz mechanism.
  
  Yamada K, Ishikawa H, Mizuno M, Shibayama N, Mizutani Y
  
  The journal of physical chemistry. B 117 12461-12468 2013年10月
  http://dx.doi.org/10.1021/jp407735t
2. Pressure-induced endocytic degradation of the Saccharomyces cerevisiae low-affinity tryptophan permease Tat1 is mediated by Rsp5 ubiquitin ligase and functionally redundant PPxY motif proteins.
  
  Suzuki A, Mochizuki T, Uemura S, Hiraki T, Abe F
  
  Eukaryotic cell 12(7) 990-997 2013年07月
  http://dx.doi.org/10.1128/EC.00049-13
3. Brevundimonas abyssalis sp. nov., a dimorphic prosthecate bacterium isolated from deep-subsea floor sediment.
  
  Tsubouchi T, Shimane Y, Usui K, Shimamura S, Mori K, Hiraki T, Tame A, Uematsu K, Maruyama T, Hatada Y
  
  International journal of systematic and evolutionary microbiology 63(Pt 6) 1987-1994 2013年06月
  http://dx.doi.org/10.1099/ijs.0.043364-0
4. Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment.
  
  Tsubouchi T, Shimane Y, Mori K, Usui K, Hiraki T, Tame A, Uematsu K, Maruyama T, Hatada Y
  
  International journal of systematic and evolutionary microbiology 63(Pt 6) 1972-1981 2013年06月
  http://dx.doi.org/10.1099/ijs.0.043596-0
5. Homopiperazine derivatives as a novel class of proteasome inhibitors with a unique mode of proteasome binding.
  
  Kikuchi J, Shibayama N, Yamada S, Wada T, Nobuyoshi M, Izumi T, Akutsu M, Kano Y, Sugiyama K, Ohki M, Park SY, Furukawa Y
  
  PloS one 8 e60649 2013年
  http://dx.doi.org/10.1371/journal.pone.0060649
2012
1. Symmetry distortion in the human hemoglobin tetramer induced by asymmetric ligation.
  
  Shibayama N
  
  FEBS letters 586 74-78 2012年01月
  http://dx.doi.org/10.1016/j.febslet.2011.11.027
2011
1. Structures and oxygen affinities of crystalline human hemoglobin C (β6 Glu->Lys) in the R and R2 quaternary structures.
  
  Shibayama N, Sugiyama K, Park SY
  
  The Journal of biological chemistry 286 33661-33668 2011年09月
  http://dx.doi.org/10.1074/jbc.M111.266056
2. MWC2状態モデルはヘモグロビンのアロステリーといかに調和しうるか
  
  柴山修哉
  
  人工血液 18(3) 102-109 2011年01月
このページの先頭へ
2010-2001
1. Protonation states of histidine and other key residues in deoxy normal human adult hemoglobin by neutron protein crystallography.
  
  Kovalevsky A, Chatake T, Shibayama N, Park SY, Ishikawa T, Mustyakimov M, Fisher SZ, Langan P, Morimoto Y
  
  Acta crystallographica. Section D, Biological crystallography 66 1144-1152 2010年11月
  http://dx.doi.org/10.1107/S0907444910025448
2. Direct determination of protonation states of histidine residues in a 2 A neutron structure of deoxy-human normal adult hemoglobin and implications for the Bohr effect.
  
  Kovalevsky AY, Chatake T, Shibayama N, Park SY, Ishikawa T, Mustyakimov M, Fisher Z, Langan P, Morimoto Y
  
  Journal of molecular biology 398 276-291 2010年04月
  http://dx.doi.org/10.1016/j.jmb.2010.03.016
3. Physicochemical properties of diheme cytochrome c4 of unknown function from Vibrio parahaemolyticus strain RIMD2210633.
  
  Akazaki H, Futami Y, Shibayama N, Shirasaki I, Nakade H, Chida H, Hakamata W, Park SY, Nishio T, Oku T
  
  Bioscience, biotechnology, and biochemistry 72 2791-2794 2008年10月
4. The structural basis for an essential subunit interaction in influenza virus RNA polymerase.
  
  Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR, Park SY
  
  Nature 454 1127-1131 2008年08月
  http://dx.doi.org/10.1038/nature07225
5. Circular dichroism study on the early folding events of beta-lactoglobulin entrapped in wet silica gels.
  
  Shibayama N
  
  FEBS letters 582 2668-2672 2008年07月
  http://dx.doi.org/10.1016/j.febslet.2008.06.047
6. Slow motion analysis of protein folding intermediates within wet silica gels.
  
  Shibayama N
  
  Biochemistry 47 5784-5794 2008年05月
  http://dx.doi.org/10.1021/bi8003582
7. Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin.
  Kovalevsky AY, Chatake T, Shibayama N, Park SY, Ishikawa T, Mustyakimov M, Fisher SZ, Langan P, Morimoto Y
  Acta Crystallogr F. 64 270-273 2008年4月
  https://doi.org/10.1107/S1744309108005137
8. Crystal structures of the clock protein EA4 from the silkworm Bombyx mori.
  
  Hiraki T, Shibayama N, Akashi S, Park SY
  
  Journal of molecular biology 377(3) 630-635 2008年03月
  http://dx.doi.org/10.1016/j.jmb.2008.01.020
9. Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography.
  
  Chatake T, Shibayama N, Park SY, Kurihara K, Tamada T, Tanaka I, Niimura N, Kuroki R, Morimoto Y
  
  Journal of the American Chemical Society 129 14840-14841 2007年12月
  http://dx.doi.org/10.1021/ja0749441
10. Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system.
  
  Hiraki T, Shibayama N, Yoon YH, Yun KM, Hamamoto T, Tame JR, Park SY
  
  Acta crystallographica. Section F, Structural biology and crystallization communications 63 734-736 2007年09月
  http://dx.doi.org/10.1107/S1744309107033854
11. 1.25 A resolution crystal structures of human haemoglobin in the oxy, deoxy and carbonmonoxy forms.
  
  Park SY, Yokoyama T, Shibayama N, Shiro Y, Tame JR
  
  Journal of molecular biology 360 690-701 2006年07月
  http://dx.doi.org/10.1016/j.jmb.2006.05.036
12. Crystal structures of unliganded and half-liganded human hemoglobin derivatives cross-linked between Lys 82beta1 and Lys 82beta2.
  
  Park SY, Shibayama N, Hiraki T, Tame JR
  
  Biochemistry 43 8711-8717 2004年07月
  http://dx.doi.org/10.1021/bi049932w
13. 光解離に伴うヘモグロビンの動きを観る
  
  柴山修哉
  
  生物物理 44(3) 108-112 2004年
  http://dx.doi.org/10.2142/biophys.44.108
14. Highly nonexponential kinetics in the early-phase refolding of proteins at low temperatures.
  
  Saigo S, Shibayama N
  
  Biochemistry 42 9669-9676 2003年08月
  http://dx.doi.org/10.1021/bi034484y
15. Direct observation of photolysis-induced tertiary structural changes in hemoglobin.
  
  Adachi S, Park SY, Tame JR, Shiro Y, Shibayama N
  
  Proceedings of the National Academy of Sciences of the United States of America 100 7039-7044 2003年06月
  http://dx.doi.org/10.1073/pnas.1230629100
16. Oxygen equilibrium properties of myoglobin locked in the liganded and unliganded conformations.
  
  Shibayama N, Saigo S
  
  Journal of the American Chemical Society 125 3780-3783 2003年04月
  http://dx.doi.org/10.1074/jbc.M205461200
17. Crystal structure of horse carbonmonoxyhemoglobin-bezafibrate complex at 1.55-A resolution. A novel allosteric binding site in R-state hemoglobin.
  
  Shibayama N, Miura S, Tame JR, Yonetani T, Park SY
  
  The Journal of biological chemistry 277 38791-38796 2002年10月
  http://dx.doi.org/10.1074/jbc.M205461200
18. The contribution of the asymmetric alpha 1beta 1 half-oxygenated intermediate to human hemoglobin cooperativity.
  
  Yun KM, Morimoto H, Shibayama N
  
  The Journal of biological chemistry 277 1878-1883 2002年01月
  http://dx.doi.org/10.1074/jbc.M108494200
19. Differences in changes of the α1-β2 subunit contacts between ligand binding to the α and β subunits of hemoglobin A: UV resonance Raman analysis using Ni-Fe hybrid hemoglobin
  
  Nagatomo, S., Nagai, M., Shibayama, N., and Kitagawa, T.
  
  Biochemistry 41 10010-10020 2002年
20. Direct observation of two distinct affinity conformations in the T state human deoxyhemoglobin
  
  Shibayama, N., and Saigo, S.
  
  FEBS Letters 492 50-53 2001年
このページの先頭へ
2000-1991
1. ゾル・ゲル法でタンパク質分子のコンホメーションを固定する
  
  柴山修哉
  
  生物物理 40(5) 346-349 2000年
  http://dx.doi.org/10.2142/biophys.40.346
2. Magnesium(II) and zinc(II)-protoporphyrin IX's stabilize the lowest oxygen affinity state of human hemoglobin even more strongly than deoxyheme
  
  Miyazaki, G. et al.
  
  Journal of Molecular Biology 292 1121-1136 1999年
3. Functional analysis of hemoglobin molecules locked in doubly liganded conformations.
  
  Shibayama, N.
  
  Journal of Molecular Biology 285 1383-1388 1999年
4. Kinetics of the allosteric transition in hemoglobin within silicate sol-gels
  
  Shibayama, N., and Saigo, S.
  
  Journal of the American Chemical Society 121 444-445 1999年
5. Asymmetric cyanomet valency hybrid hemoglobin, (α+CN-β+CN-)(αβ): The Issue of valency exchange
  
  Shibayama, N., Morimoto, H., and Saigo, S.
  
  Biochemistry 37 6221-6228 1998年
6. Rate constants for O2 and CO binding to the alpha and beta subunits within the R and T states of human hemoglobin
  
  Unzai, S., Eich, R., Shibayama, N., Olson, J. S., and Morimoto, H.
  
  Journal of Biological Chemistry 273 23150-23159 1998年
7. 作用反作用の法則は誤解されている
  
  青野修, 西郷敏, 原田三男, 柴山修哉
  
  物理教育 45(6) 328-329 1997年12月
8. Reexamination of the hyper thermodynamic stability of asymmetric cyanomet valency hybrid hemoglobin, (α+CN-β+CN-)(αβ): No preferentially populating asymmetric hybrid at equilibrium
  
  Shibayama, N., Morimoto, H., and Saigo, S.
  
  Biochemistry 36 4375-4381 1997年
9. Oxygen equilibrium properties of chromium(III)-iron(II) hybrid hemoglobins
  
  Unzai, S., Hori, H., Miyazaki, G., Shibayama, N., and Morimoto, H.
  
  Journal of Biological Chemistry 271 12451-12456 1996年
10. Oxygen equilibrium and electron paramagnetic resonance studies on copper(II)-iron(II) hybrid hemoglobins at room temperature
  
  Shibayama, N. et al.
  
  FEBS Letters 372 126-130 1995年
11. Mechanism of ligand binding to Ni(II)-Fe(II) hybrid hemoglobins
  
  Shibayama, N., Yonetani, T., Regan, R. M., and Gibson, Q. H.
  
  Biochemistry 34 14658-14667 1995年
12. Oxygen equilibrium properties of nickel(II)-iron(II) hybrid hemoglobins cross-linked between 82β1 and 82β2 lysyl residues by bis(3,5-dibromosalicyl)fumarate: Determination of the first two-step microscopic Adair constants for human hemoglobin
  
  Shibayama, N., Imai, K., Morimoto, H., and Saigo, S.
  
  Biochemistry 34 4773-4780 1995年
13. Fixation of the quaternary structures of human adult haemoglobin by encapsulation in transparent porous silica gels
  
  Shibayama, N., and Saigo, S.
  
  Journal of Molecular Biology 251 203-209 1995年
14. 大学物理教育をめぐってその2 医歯薬系の基礎物理自治医科大学における物理学の新しい教育課程
  
  青野修, 西郷敏, 原田三男, 柴山修哉
  
  物理教育 42(3) 307-310 1994年09月
15. X-ray absorption spectroscopic studies of a transient intermediate in the reaction of cyanide metmyoglobin with dithionite by using rapid freezing
  
  Saigo, S., Hashimoto, H., Shibayama, N., Nomura, M., and Nagamura, T.
  
  Biochim. Biophys. Acta. 1202 99-106 1993年
16. Rapid-freeze XAFS characterization of kinetic intermediates of metalloproteins
  
  Saigo, S., Hashimoto, H., Shibayama, N., Nomura, M., and Nagamura, T.
  
  Jpn. J. Appl. Phys. 32 538-540 1993年
17. Oxygen equilibrium properties of asymmetric nickel(II)-iron(II) hybrid hemoglobin
  
  Shibayama, N., Imai, K., Morimoto, H., and Saigo, S.
  
  Biochemistry 32 8792-8798 1993年
18. Polymerization and solubility of Ni(II)-Fe(II) hybrid Hb S
  
  Adachi, K., Kim, J., and Shibayama, N.
  
  Biochim. Biophys. Acta. 1079 268-272 1991年
19. Oxygen equilibrium properties of highly purified human adult hemoglobin cross-linked between 82β1 and 82β2 lysyl residues by bis(3,5-dibromosalicyl)fumarate
  
  Shibayama, N. et al.
  
  Biochemistry 30 8158-8165 1991年
1990-
1. Structure of deoxy-quaternary haemoglobin with liganded β subunits
  
  Luisi, B., Liddington, B., Fermi, G., and Shibayama, N.
  
  Journal of Molecular Biology 214 7-14 1990年
2. Polymerization of partially liganded hemoglobin S
  
  Adachi, K., Kim, J., and Shibayama, N.
  
  Sickle Cell Disease 565 356-357 1989年
3. Structure of haemoglobin in the deoxy quaternary state with ligand bound at the α haems.
  
  Luisi, B., and Shibayama, N.
  
  Journal of Molecular Biology 206 723-736 1989年

このページの先頭へ

自治医科大学　生物物理学部門

〒329-0498
栃木県下野市薬師寺3311-1

自治医科大学医学部生理学講座生物物理学部門

研究成果PUBLICATIONS

発表論文

contents

バナースペース

自治医科大学　生物物理学部門

自治医科大学 医学部 生理学講座 生物物理学部門

研究成果PUBLICATIONS

発表論文

contents

バナースペース

自治医科大学 生物物理学部門

自治医科大学医学部生理学講座生物物理学部門

自治医科大学　生物物理学部門