We aim to elucidate molecular mechanisms regulating cardiomyocyte maturation.
With an efficient, directed cardiac differentiation method, we are able to obtain cardiomyocytes from pluripotent stem cells. However, such cardiomyocytes were immature as fetal cardiomyocytes, which limits the potential of stem cell derived cardiomyocytes being a platform to study human heart diseases.
We will elucidate the molecular mechanisms and provide a platform to recaptulate human heart diseases in vitro.
We are working on to model cardiac myopathy and malformation in vivo and/or in vitro.
In a collabolation with Drs. Koitabashi (Gunma U), Nishizono (Max Planck Florida), and other researchers of Jichi Medical Unviersity, we are generating animal models harvoring SNVs that found in congenital heart diseases and cardiac myopathy.
As a part of an AMED-sponsored project, we are establishing a number of patient-derived iPS cell lines for mitochondrial diseases and neural disorders in collabolation with Dr. Murayama (Chiba Children's Hospital) and our pediatrics department.
To analyze the physiological function of iPS cell-derived cardiomyocytes, we are developing new tools and techniques for sarcomere shortining with live-imaging, which may facilitate the cardiomyopathy study.
These studies will provide novel perspectives in heart diseases.
2004: M.D., Hokkaido University
2011: Ph.D, Kyoto University
In 2016, he joined to the division as a lecturer. In 2017, he was promoted to Associate Professor.
2012: M.D., Kanazawa University.
2022: Ph.D., Jichi Medical University
After residency at JICHI Medical University, he entered Ph.D. program at pediatrics department, JICHI Medical University in 2018.
He joined our lab to study VUS, related to congenital heart diseases, using mouse models and stem cell biology.
In 2022, he was awarded Ph.D. from JICHI Medical University and promoted to Assistant Professor of the pediatrics department, Jichi Meidlca University.
He contineus his gene thepary studies here as a part-time.
2017: Ph.D., Tokyo University of Pharmacy and Life Sciences.
After being a postdoc for Tokyo University of Pharmacy and Life Sciences and Johns Hopkins University, he joined as a postdoc in 2021.
Ph.D Student. He graduated from Chulalongkorn University, Thailand and joined our lab in 2016 as a Ph.D. student (JMU Special International Graduate Student Program). In 2020, Nawin was awarded a Ph.D.
2010: D.Ds., Padjajaran University.
2013: M.Biotech., Gadjah Mada University.
2019: Ph.D., The University of Tokyo.
After being a postdoc at the University of Tokyo, he joined our lab as a postdoc in 2022.
2017: M.D., The University of Khartoum.
After one year of Internship at Ministry of Health Hospital in Sudan, she joined our lab as a research student in July, 2019 and continues as a graduate student from 2020
Byambaa S, Uosaki H, Hara H, Nagao Y, Abe T, Shibata H, Nureki O, Ohmori T, Hanazono Y. Generation of Novel Il2rg-Knockout Mice with CRISPR/Cas9. Exp Anim. 2020 Apr 24;69(2):189-198. doi: 10.1538/expanim.19-0120.
Nishizono H, Darwish M, Uosaki H, Masuyama N, Seki M, Abe H, Yachie N, Yasuda R. The Use of Freeze-thawed Embryos for the High-efficient Production of Genetically Modified Mice. J Vis Exp. 2020 Apr;158:e60808 doi: 10.3791/60808.
Ahmed RE, Anzai T, Chanthra N, Uosaki H. A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-derived Cardiomyocytes. Front Cell Dev Biol. 2020 Mar 19; doi: 10.3389/fcell.2020.00178. Review
Chanthra N, Abe T, Miyamoto M, Sekiguchi K, Kwon C, Hanazono Y, Uosaki H. A Novel Fluorescent Reporter System Identifies Laminin-511/521 as Potent Regulators of Cardiomyocyte Maturation. Sci Rep. 2020 Mar 6; doi: 10.1038/s41598-020-61163-3
Darwish M, Nishizono H, Uosaki H, Sawada H, Sadahiro T, Ieda M, Takao K. Rapid and high-efficient generation of mutant mice using freeze-thawed embryos of the C57BL/6J strain. J Neurosci Methods. 2019 Apr 1;317:149-156. doi: 10.1016/j.jneumeth.2019.01.010.
Sadahiro T, Isomi M, Muraoka N, Kojima H, Haginiwa S, Kurotsu S, Tamura F, Tani H, Tohyama S, Fujita J, Miyoshi H, Kawamura Y, Goshima N, Iwasaki YW, Murano K, Saito K, Oda M, Andersen P, Kwon C, Uosaki H, Nishizono H, Fukuda K, Ieda M. Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification. Cell Stem Cell. 2018 Sep 6;23(3):382-395.e5 doi: 10.1016/j.stem.2018.07.001.
Hara H, Shibata H, Nakano K, Abe T, Uosaki H, Ohnuki T, Hishikawa S, Kunita S, Watanabe M, Nureki O, Nagashima H, Hanazono Y. Production and rearing of germ-free X-SCID pigs. Exp Anim. 2018 May 10;67(2):139-146. doi: 10.1538/expanim.17-0095.
Cho GS, Lee DI, Tampakakis E, Murphy S, Andersen P, Uosaki H, Chelko S, Chakir K, Hong I, Seo K, Chen HV, Chen X, Basso C, Houser SR, Tomaselli GF, O' Rourke B, Judge DP, Kass DA, and Kown C. Neonatal transplantation confers maturation of PSC-derived cardiomyocytes conducive to modeling cardiomyopathy. Cell Rep. 2017 Jan 10;18(2):571-582. doi: 10.1016/j.celrep.2016.12.040.