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自治医科大学医学部薬理学講座 分子薬理学部門



  1. Sakai, N., Saito, Y., Fujiwara, Y., Shiraki, T., Imanishi, Y., Koshimizu, T.A., and Shibata, K., Identification of protein arginine N-methyltransferase 5 (PRMT5) as a novel interacting protein with the tumor suppressor protein RASSF1A. Biochem Biophys Res Commun, 2015. Accepted.
  2. Kashiwazaki, A., Fujiwara, Y., Tsuchiya, H., Sakai, N., Shibata, K., and Koshimizu, T.A., Subcellular localization and internalization of the vasopressin V receptor. Eur J Pharmacol, 2015. 765: p. 291-299.
  3. Ishikura, T., Suzuki, H., Shoguchi, K., Koreeda, Y., Aritomi, T., Matsuura, T., Yoshimura, M., Ohkubo, J.I., Maruyama, T., Kawasaki, M., Ohnishi, H., Sakai, A., Mizuno, A., Suzuki, M., and Ueta, Y., Possible involvement of TRPV1 and TRPV4 in nociceptive stimulation- induced nocifensive behavior and neuroendocrine response in mice. Brain Res Bull, 2015. 118: p. 7-16.
  4. Arai, K., Kashiwazaki, A., Fujiwara, Y., Tsuchiya, H., Sakai, N., Shibata, K., and Koshimizu, T.A., Pharmacological lineage analysis revealed the binding affinity of broad-spectrum substance P antagonists to receptors for gonadotropin-releasing peptide. Eur J Pharmacol, 2015. 749: p. 98-106.
  5. Aijima, R., Wang, B., Takao, T., Mihara, H., Kashio, M., Ohsaki, Y., Zhang, J.Q., Mizuno, A., Suzuki, M., Yamashita, Y., Masuko, S., Goto, M., Tominaga, M., and Kido, M.A., The thermosensitive TRPV3 channel contributes to rapid wound healing in oral epithelia. FASEB J, 2015. 29(1): p. 182-92.
  6. Tsuchiya, H., Sato, J., Tsuda, H., Fujiwara, Y., Yamada, T., Fujimura, A., and Koshimizu, T.A., Serum amyloid A upsurge precedes standard biomarkers of hepatotoxicity in ritodrine-injected mice. Toxicology, 2013. 305: p. 79-88.
  7. Tamaki, Y., Iwanaga, Y., Niizuma, S., Kawashima, T., Kato, T., Inuzuka, Y., Horie, T., Morooka, H., Takase, T., Akahashi, Y., Kobuke, K., Ono, K., Shioi, T., Sheikh, S.P., Ambartsumian, N., Lukanidin, E., Koshimizu, T.A., Miyazaki, S., and Kimura, T., Metastasis-associated protein, S100A4 mediates cardiac fibrosis potentially through the modulation of p53 in cardiac fibroblasts. J Mol Cell Cardiol, 2013. 57: p. 72-81.
  8. Masuki, S., Sumiyoshi, E., Koshimizu, T.A., Qian, J., Higuchi, K., Tsujimoto, G., and Nose, H., Voluntary locomotion linked with cerebral activation is mediated by vasopressin V1a receptors in free-moving mice. J Physiol, 2013. 591(Pt 14): p. 3651-65.
  9. Kumazaki, M., Ando, H., Kakei, M., Ushijima, K., Taniguchi, Y., Yoshida, M., Yamato, S., Washino, S., Koshimizu, T.A., and Fujimura, A., alpha-Lipoic acid protects against arsenic trioxide-induced acute QT prolongation in anesthetized guinea pigs. Eur J Pharmacol, 2013. 705(1-3): p. 1-10.
  10. Tsuchiya, H., Ushijima, K., Fujiwara, Y., Fujimura, A., and Koshimizu, T.A., Chronic ritodrine treatment induces refractoriness of glucose-lowering beta2 adrenoceptor signal in female mice. Regul Toxicol Pharmacol, 2012. 62(3): p. 561-7.
  11. Koshimizu, T.A., Nakamura, K., Egashira, N., Hiroyama, M., Nonoguchi, H., and Tanoue, A., Vasopressin V1a and V1b receptors: from molecules to physiological systems. Physiol Rev, 2012. 92(4): p. 1813-64.
  12. Ichimura, A., Hirasawa, A., Poulain-Godefroy, O., Bonnefond, A., Hara, T., Yengo, L., Kimura, I., Leloire, A., Liu, N., Iida, K., Choquet, H., Besnard, P., Lecoeur, C., Vivequin, S., Ayukawa, K., Takeuchi, M., Ozawa, K., Tauber, M., Maffeis, C., Morandi, A., Buzzetti, R., Elliott, P., Pouta, A., Jarvelin, M.R., Korner, A., Kiess, W., Pigeyre, M., Caiazzo, R., Van, H.W., Van, G.L., Horber, F., Balkau, B., Levy-Marchal, C., Rouskas, K., Kouvatsi, A., Hebebrand, J., Hinney, A., Scherag, A., Pattou, F., Meyre, D., Koshimizu, T.A., Wolowczuk, I., Tsujimoto, G., and Froguel, P., Dysfunction of lipid sensor GPR120 leads to obesity in both mouse and human. Nature, 2012. 483(7389): p. 350-4.
  13. Fujiwara, Y., Tanoue, A., Tsujimoto, G., and Koshimizu, T.A., The roles of V1a vasopressin receptors in blood pressure homeostasis: a review of studies on V1a receptor knockout mice. Clinical and experimental nephrology, 2012. 16(1): p. 30-4.
  14. Kumazaki, M., Ando, H., Sasaki, A., Koshimizu, T.A., Ushijima, K., Hosohata, K., Oshima, Y., and Fujimura, A., Protective effect of alpha-lipoic acid against arsenic trioxide-induced acute cardiac toxicity in rats. Journal of pharmacological sciences, 2011. 115(2): p. 244-8.
  15. Fujiwara, Y., Tsuchiya, H., Sakai, N., Shibata, K., Fujimura, A., and Koshimizu, T.A., Proximal tubules and podocytes are toxicity targets of bucillamine in a mouse model of drug-induced kidney injury. Eur J Pharmacol, 2011. 670(1): p. 208-15.
  16. Muto, S., Hata, M., Taniguchi, J., Tsuruoka, S., Moriwaki, K., Saitou, M., Furuse, K., Sasaki, H., Fujimura, A., Imai, M., Kusano, E., Tsukita, S., and Furuse, M., Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules. Proc Natl Acad Sci U S A, 2010. 107(17): p. 8011-6.
  17. Koshimizu, T.A., Tsuchiya, H., Tsuda, H., Fujiwara, Y., Shibata, K., Hirasawa, A., Tsujimoto, G., and Fujimura, A., Inhibition of heat shock protein 90 attenuates adenylate cyclase sensitization after chronic morphine treatment. Biochem Biophys Res Commun, 2010. 392(4): p. 603-7.
  18. Koshimizu, T.A., Fujiwara, Y., Sakai, N., Shibata, K., and Tsuchiya, H., Oxytocin stimulates expression of a noncoding RNA tumor marker in a human neuroblastoma cell line. Life Sci, 2010. 86(11-12): p. 455-60.
  19. Ushijima, K., Tsuruoka, S., Tsuda, H., Hasegawa, G., Obi, Y., Kaneda, T., Takahashi, M., Maekawa, T., Sasaki, T., Koshimizu, T.A., and Fujimura, A., Cranberry juice suppressed the diclofenac metabolism by human liver microsomes, but not in healthy human subjects. Br J Clin Pharmacol, 2009. 68(2): p. 194-200.
  20. Stojilkovic, S.S., He, M.L., Koshimizu, T.A., Balik, A., and Zemkova, H., Signaling by purinergic receptors and channels in the pituitary gland. Mol Cell Endocrinol, 2009. 314(2): p. 184-91.
  21. Koshimizu, T.A. and Tsujimoto, G., New topics in vasopressin receptors and approach to novel drugs: vasopressin and pain perception. J Pharmacol Sci, 2009. 109(1): p. 33-7.
  22. Kojima, Y., Sasaki, S., Oda, N., Koshimizu, T.A., Hayashi, Y., Kiniwa, M., Tsujimoto, G., and Kohri, K., Prostate growth inhibition by subtype-selective alpha(1)-adrenoceptor antagonist naftopidil in benign prostatic hyperplasia. Prostate, 2009. 69(14): p. 1521-8.
  23. Hara, T., Hirasawa, A., Sun, Q., Sadakane, K., Itsubo, C., Iga, T., Adachi, T., Koshimizu, T.A., Hashimoto, T., Asakawa, Y., and Tsujimoto, G., Novel selective ligands for free fatty acid receptors GPR120 and GPR40. Naunyn Schmiedebergs Arch Pharmacol, 2009. 380(3): p. 247-55.
  24. Hara, T., Hirasawa, A., Sun, Q., Koshimizu, T.A., Itsubo, C., Sadakane, K., Awaji, T., and Tsujimoto, G., Flow cytometry-based binding assay for GPR40 (FFAR1; free fatty acid receptor 1). Mol Pharmacol, 2009. 75(1): p. 85-91.
  25. Aoyagi, T., Koshimizu, T.A., and Tanoue, A., Vasopressin regulation of blood pressure and volume: findings from V1a receptor-deficient mice. Kidney Int, 2009. 76(10): p. 1035-9.
  26. Tsuchiya, H., Oka, T., Nakamura, K., Ichikawa, A., Saper, C.B., and Sugimoto, Y., Prostaglandin E2 attenuates preoptic expression of GABAA receptors via EP3 receptors. J Biol Chem, 2008. 283(16): p. 11064-71.
  27. Tanaka, T., Yano, T., Adachi, T., Koshimizu, T.A., Hirasawa, A., and Tsujimoto, G., Cloning and characterization of the rat free fatty acid receptor GPR120: in vivo effect of the natural ligand on GLP-1 secretion and proliferation of pancreatic beta cells. Naunyn Schmiedebergs Arch Pharmacol, 2008. 377(4-6): p. 515-22.
  28. Tanaka, T., Katsuma, S., Koshimizu, T.A., Hirasawa, A., and Tsujimoto, G., Free fatty acids induce cholecystokinin secretion through GPR120. Naunyn Schmiedebergs Arch Pharmacol, 2008. 377(4-6): p. 523-7.
  29. Mizuno, A., [TRP channels and mechanical stress]. Clin Calcium, 2008. 18(9): p. 1304-11.
  30. Hirasawa, A., Itsubo, C., Sadakane, K., Hara, T., Shinagawa, S., Koga, H., Nose, H., Koshimizu, T.A., and Tsujimoto, G., Production and characterization of a monoclonal antibody against GPR40 (FFAR1; free fatty acid receptor 1). Biochem Biophys Res Commun, 2008. 365(1): p. 22-8.
  31. Taniguchi, J., Tsuruoka, S., Mizuno, A., Sato, J., Fujimura, A., and Suzuki, M., TRPV4 as a flow sensor in flow-dependent K+ secretion from the cortical collecting duct. Am J Physiol Renal Physiol, 2007. 292(2): p. F667-73.
  32. Myoumoto, A., Nakatani, K., Koshimizu, T.A., Matsubara, H., Adachi, S., and Tsujimoto, G., Glucocorticoid-induced granzyme A expression can be used as a marker of glucocorticoid sensitivity for acute lymphoblastic leukemia therapy. J Hum Genet, 2007. 52(4): p. 328-33.
  33. Koshimizu, T.A., Tanoue, A., and Tsujimoto, G., Clinical implications from studies of alpha1 adrenergic receptor knockout mice. Biochem Pharmacol, 2007. 73(8): p. 1107-12.
  34. Taniguchi, J. and Imai, M., Computer analysis of the significance of the effective osmolality for urea across the inner medullary collecting duct in the operation of a single effect for the counter-current multiplication system. Clin Exp Nephrol, 2006. 10(4): p. 236-43.
  35. Koshimizu, T.A. and Tsujimoto, G., Functional Role of Spliced Cytoplasmic Tails in P2X(2)-Receptor-Mediated Cellular Signaling. J Pharmacol Sci, 2006. 101(4): p. 261-6.
  36. Koshimizu, T.A., Nasa, Y., Tanoue, A., Oikawa, R., Kawahara, Y., Kiyono, Y., Adachi, T., Tanaka, T., Kuwaki, T., Mori, T., Takeo, S., Okamura, H., and Tsujimoto, G., V1a vasopressin receptors maintain normal blood pressure by regulating circulating blood volume and baroreflex sensitivity. Proc Natl Acad Sci U S A, 2006. 103(20): p. 7807-12.
  37. Koshimizu, T.A., Kretschmannova, K., He, M.L., Ueno, S., Tanoue, A., Yanagihara, N., Stojilkovic, S.S., and Tsujimoto, G., Carboxyl-terminal splicing enhances physical interactions between the cytoplasmic tails of purinergic P2X receptors. Mol Pharmacol, 2006. 69(5): p. 1588-98.
  38. Adachi, T., Tanaka, T., Takemoto, K., Koshimizu, T.A., Hirasawa, A., and Tsujimoto, G., Free fatty acids administered into the colon promote the secretion of glucagon-like peptide-1 and insulin. Biochem Biophys Res Commun, 2006. 340(1): p. 332-7.
  39. Yamada, M., Katsuma, S., Adachi, T., Hirasawa, A., Shiojima, S., Kadowaki, T., Okuno, Y., Koshimizu, T.A., Fujii, S., Sekiya, Y., Miyamoto, Y., Tamura, M., Yumura, W., Nihei, H., Kobayashi, M., and Tsujimoto, G., Inhibition of protein kinase CK2 prevents the progression of glomerulonephritis. Proc Natl Acad Sci U S A, 2005. 102(21): p. 7736-41.
  40. Stojilkovic, S.S., Tomic, M., He, M.L., Yan, Z., Koshimizu, T.A., and Zemkova, H., Molecular Dissection of Purinergic P2X Receptor Channels. Ann N Y Acad Sci, 2005. 1048: p. 116-30.
  41. Hosoda, C., Tanoue, A., Shibano, M., Tanaka, Y., Hiroyama, M., Koshimizu, T.A., Cotecchia, S., Kitamura, T., Tsujimoto, G., and Koike, K., Correlation between vasoconstrictor roles and mRNA expression of alpha1-adrenoceptor subtypes in blood vessels of genetically engineered mice. Br J Pharmacol, 2005. 146(3): p. 456-66.
  42. Hosoda, C., Koshimizu, T.A., Tanoue, A., Nasa, Y., Oikawa, R., Tomabechi, T., Fukuda, S., Shinoura, H., Oshikawa, S., Takeo, S., Kitamura, T., Cotecchia, S., and Tsujimoto, G., Two alpha1-adrenergic receptor subtypes regulating the vasopressor response have differential roles in blood pressure regulation. Mol Pharmacol, 2005. 67(3): p. 912-22.
  43. Zemkova, H., He, M.L., Koshimizu, T.A., and Stojilkovic, S.S., Identification of ectodomain regions contributing to gating, deactivation, and resensitization of purinergic P2X receptors. J Neurosci, 2004. 24(31): p. 6968-78.
  44. Todaka, H., Taniguchi, J., Satoh, J., Mizuno, A., and Suzuki, M., Warm temperature-sensitive transient receptor potential vanilloid 4 (TRPV4) plays an essential role in thermal hyperalgesia. J Biol Chem, 2004. 279(34): p. 35133-8.
  45. Tanoue, A., Koshimizu, T.A., Tsujimoto, G., Nakata, H., Hirose, S., Fukuzawa, T., Abe, J., and Kurose, H., Heterogeneity of G protein-coupled receptor generated by post-translational mechanisms and its clinical meanings. Nippon Yakurigaku Zasshi, 2004. 124(4): p. 235-43.
  46. Tanoue, A., Ito, S., Honda, K., Oshikawa, S., Kitagawa, Y., Koshimizu, T.A., Mori, T., and Tsujimoto, G., The vasopressin V1b receptor critically regulates hypothalamic-pituitary-adrenal axis activity under both stress and resting conditions. J Clin Invest, 2004. 113(2): p. 302-9.
  47. Suzuki, M. and Mizuno, A., A novel human Cl(-) channel family related to Drosophila flightless locus. J Biol Chem, 2004. 279(21): p. 22461-8.
  48. Oshikawa, S., Tanoue, A., Koshimizu, T.A., Kitagawa, Y., and Tsujimoto, G., Vasopressin stimulates insulin release from islet cells through V1b receptors: a combined pharmacological/knockout approach. Mol Pharmacol, 2004. 65(3): p. 623-9.
  49. Koshimizu, T.A., Tsujimoto, G., Hirasawa, A., Kitagawa, Y., and Tanoue, A., Carvedilol selectively inhibits oscillatory intracellular calcium changes evoked by human alpha1D- and alpha1B-adrenergic receptors. Cardiovasc Res, 2004. 63(4): p. 662-72.
  50. Tanoue, A., Koshimizu, T.A., Shibata, K., Nasa, Y., Takeo, S., and Tsujimoto, G., Insights into alpha(1) adrenoceptor function in health and disease from transgenic animal studies. Trends Endocrinol Metab, 2003. 14(3): p. 107-13.
  51. Suzuki, M., Watanabe, Y., Oyama, Y., Mizuno, A., Kusano, E., Hirao, A., and Ookawara, S., Localization of mechanosensitive channel TRPV4 in mouse skin. Neurosci Lett, 2003. 353(3): p. 189-92.
  52. Suzuki, M., Mizuno, A., Kodaira, K., and Imai, M., Impaired pressure sensation in mice lacking TRPV4. J Biol Chem, 2003. 278(25): p. 22664-8.
  53. Suzuki, M., Hirao, A., and Mizuno, A., Microtubule-associated [corrected] protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4). J Biol Chem, 2003. 278(51): p. 51448-53.
  54. Shibata, K., Katsuma, S., Koshimizu, T.A., Shinoura, H., Hirasawa, A., Tanoue, A., and Tsujimoto, G., alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP-dependent mechanism involving p27Kip1. J Biol Chem, 2003. 278(1): p. 672-8.
  55. Mizuno, A., Matsumoto, N., Imai, M., and Suzuki, M., Impaired osmotic sensation in mice lacking TRPV4. Am J Physiol Cell Physiol, 2003. 285(1): p. C96-101.
  56. He, M.L., Zemkova, H., Koshimizu, T.A., Tomic, M., and Stojilkovic, S.S., Intracellular calcium measurements as a method in studies on activity of purinergic P2X receptor channels. Am J Physiol Cell Physiol, 2003. 285(2): p. C467-79.
  57. Tanoue, A., Nasa, Y., Koshimizu, T.A., Shinoura, H., Oshikawa, S., Kawai, T., Sunada, S., Takeo, S., and Tsujimoto, G., The alpha(1D)-adrenergic receptor directly regulates arterial blood pressure via vasoconstriction. J Clin Invest, 2002. 109(6): p. 765-75.
  58. Tanoue, A., Koshimizu, T.A., and Tsujimoto, G., Transgenic studies of alpha(1)-adrenergic receptor subtype function. Life Sci, 2002. 71(19): p. 2207-15.
  59. Tanoue, A., Koshimizu, T.A., Tsuchiya, M., Ishii, K., Osawa, M., Saeki, M., and Tsujimoto, G., Two novel transcripts for human endothelin B receptor produced by RNA editing/alternative splicing from a single gene. J Biol Chem, 2002. 277(36): p. 33205-12.
  60. Tanoue, A., Koba, M., Miyawaki, S., Koshimizu, T.A., Hosoda, C., Oshikawa, S., and Tsujimoto, G., Role of the alpha1D-adrenegric receptor in the development of salt-induced hypertension. Hypertension, 2002. 40(1): p. 101-6.
  61. Koshimizu, T.A., Ueno, S., Tanoue, A., Yanagihara, N., Stojilkovic, S.S., and Tsujimoto, G., Heteromultimerization modulates P2X receptor functions through participating extracellular and C-terminal subdomains. J Biol Chem, 2002. 277(49): p. 46891-9.
  62. Koshimizu, T.A., Komori, S., Ishihara, T., Kohno, I., Umetani, K., Sawanobori, T., Ijiri, H., and Tamura, K., Restored atrial excitability after late recanalization in a patient with atrial standstill and acute myocardial infarction. Pacing Clin Electrophysiol, 2002. 25(2): p. 217-9.
  63. He, M.L., Koshimizu, T.A., Tomic, M., and Stojilkovic, S.S., Purinergic P2X(2) receptor desensitization depends on coupling between ectodomain and C-terminal domain. Mol Pharmacol, 2002. 62(5): p. 1187-97.
  64. Stojilkovic, S.S. and Koshimizu, T.A., Signaling by extracellular nucleotides in anterior pituitary cells. Trends Endocrinol Metab, 2001. 12(5): p. 218-25.
  65. Andric, S.A., Kostic, T.S., Tomic, M., Koshimizu, T.A., and Stojilkovic, S.S., Dependence of soluble guanylyl cyclase activity on calcium signaling in pituitary cells. J Biol Chem, 2001. 276(1): p. 844-9.
  66. Stojilkovic, S.S., Tomic, M., Van Goor, F., and Koshimizu, T.A., Expression of purinergic P2X2 receptor-channels and their role in calcium signaling in pituitary cells. Biochem Cell Biol, 2000. 78(3): p. 393-404.
  67. Stojilkovic, S.S., Tomic, M., Koshimizu, T.A., and Van Goor, F., Calcium ions as intracellular messengers: pathways and action, in Molecular Regulation, Conn, M., Means, A. R., Editor. 2000, Humana press: Totowa, NJ.
  68. Koshimizu, T.A., Van Goor, F., Tomic, M., Wong, A.O., Tanoue, A., Tsujimoto, G., and Stojilkovic, S.S., Characterization of calcium signaling by purinergic receptor-channels expressed in excitable cells. Mol Pharmacol, 2000. 58(5): p. 936-45.
  69. Koshimizu, T.A., Tomic, M., Wong, A.O., Zivadinovic, D., and Stojilkovic, S.S., Characterization of purinergic receptors and receptor-channels expressed in anterior pituitary cells. Endocrinology, 2000. 141(11): p. 4091-9
  70. Tomic, M., Zivadinovic, D., Van Goor, F., Yuan, D., Koshimizu, T., and Stojilkovic, S.S. Expression of Ca(2+)-mobilizing endothelin(A) receptors and their role in the control of Ca(2+) influx and growth hormone secretion in pituitary somatotrophs. J Neurosci 19:7721-7731. (1999)
  71. Tomic, M., Koshimizu, T., Yuan, D., Andric, S.A., Zivadinovic, D., and Stojilkovic, S.S. Characterization of a plasma membrane calcium oscillator in rat pituitary somatotrophs. J Biol Chem 274:35693-35702. (1999)
  72. Koshimizu, T., Koshimizu, M., and Stojilkovic, S.S. Contributions of the C-terminal domain to the control of P2X receptor desensitization. J Biol Chem 274:37651-37657. (1999)
  73. Katzur, A.C., Koshimizu, T., Tomic, M., Schultze-Mosgau, A., Ortmann, O., and Stojilkovic, S.S. Expression and responsiveness of P2Y2 receptors in human endometrial cancer cell lines. J Clin Endocrinol Metab 84:4085-4091. (1999)
  74. Koshimizu, T., Tomic, M., Van Goor, F., and Stojilkovic, S.S. Functional role of alternative splicing in pituitary P2X2 receptor-channel activation and desensitization. Mol Endocrinol 12:901-913. (1998)
  75. Koshimizu, T., Tomic, M., Koshimizu, M., and Stojilkovic, S.S. Identification of amino acid residues contributing to desensitization of the P2X2 receptor channel. J Biol Chem 273:12853-12857. (1998)
  76. Koshimizu, T., Tsujimoto, G., Ono, K., Masaki, T., and Sakamoto, A. Truncation of the receptor carboxyl terminus impairs membrane signaling but not ligand binding of human ETB endothelin receptor. Biochem Biophys Res Commun 217:354-362. (1995)