坂下英司

【職名】講師

【出身大学】神戸大学理学部

【学位】博士 (理学) (神戸大学) 1999年3月

【職歴】1999年4月-2001年1月 University of Miami School of Medicine, Postdoctoral Fellow

【着任年月日】
2001年2月1日 助手(生化学講座)、
2006年2月1日 学内講師(生化学講座)
2008年6月1日 講師(生化学講座)
2013年4月1日 大学院講師（併任）

【専門分野】分子生物学

【研究活動】

  • 研究テーマ:
    • Researchmap Link

    以下のリンクをクリックしてください: researchmap

    関節リウマチ治療薬の休薬指標となるバイオマーカー探索
    刺激によるスプライシング因子の核内再分配の研究
    筋特異的な選択的スプライシングの調節機構の研究
  • 研究内容キーワード:
    • 関節リウマチ、バイオマーカー、mRNA前駆体スプライシング、スプライシング因子、核内再編成、筋分化、RNA結合タンパク質

【教育活動】

  • 「生化学」「生化学実習 」「生命科学1 」(分担)

【原著論文】

  • Sakashita E, Nagatani K, Endo H, Minota S. "Biomarker combination predicting imminent relapse after discontinuation of biological drugs in patients with rheumatoid arthritis in remission." PLOS ONE 19(3) e0299450-e0299450 2024. [Pubmed]
  • Tominaga K, Sakashita E, Kasashima K, Kuroiwa K, Nagao Y, Iwamori N, Endo H. "Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells." International Journal of Molecular Sciences 24(3) 2113-2113 2023. [Pubmed]
  • Sakashita E, Nagatani K, Endo K, Minota S. "Serum level of IFNβ distinguishes early from late relapses after biologics withdrawal in rheumatoid arthritis." Scientific Reports 12(1) 16547-16547 2022. [Pubmed]
  • Nagatani K, Sakashita E, Endo H, Minota S. "A novel multi-biomarker combination predicting relapse from long-term remission after discontinuation of biological drugs in rheumatoid arthritis." Sci. Rep., 2021;11:20771. [Pubmed]
  • Kuroda R, Tominaga K, Kasashima K, Kuroiwa K, Sakashita E, Hayakawa H, Kouki T, Ohno N, Kawai K, Endo H. "Loss of mitochondrial transcription factor A in neural stem cells leads to immature brain development and triggers the activation of the integral stress response in vivo." PLos One, 2021;16:e0255355. [Pubmed]
  • Miyata S, Tominaga K, Sakashita E, Urabe M, Onuki Y, Gomi A, Yamaguchi T, Mieno M, Mizukami H, Kume A, Ozawa K, Watanabe E, Kawai K, Endo H. "Comprehensive Metabolomic Analysis of IDH1 R132H Clinical Glioma Samples Reveals Suppression of β-oxidation Due to Carnitine Deficiency." Sci. Rep., 2019;9:9787. [Pubmed]
  • Mashiko T, Sakashita E, Kasashima K, Tominaga K, Kuroiwa K, Nozaki Y, Matsuura T, Hamamoto T, Endo H. "Developmentally-regulated RNA-binding Protein 1 (Drb1)/RNA-binding Motif Protein 45 (RBM45), a Nuclear-cytoplasmic Trafficking Protein, Forms TAR DNA-binding Protein 43 (TDP-43)-mediated Cytoplasmic Aggregates." J. Biol. Chem. 2016;291:14996-15007. [Pubmed]
  • Tetsuka S, Tominaga K, Ohta E, Kuroiwa K, Sakashita E, Kasashima K, Hamamoto T, Namekawa M, Morita M, Natsui S, Morita T, Tanaka K, Takiyama Y, Nakano I, Endo H. "Paraneoplastic cerebellar degeneration associated with an onconeural antibody against creatine kinase, brain-type." J. Neurol. Sci. 2013;335:48-57. [Pubmed]
  • Akimoto C, Sakashita E, Kasashima K, Kuroiwa K, Tominaga K, Hamamoto T, Endo H. "Translational repression of the McKusick-Kaufman syndrome transcript by unique upstream open reading frames encoding mitochondrial proteins with alternative polyadenylation sites." Biochim. Biophys. Acta. 2013;1830:2728-38. [Pubmed]
  • Sakashita E and Endo H. "SR and SR-related proteins redistribute to segregated fibrillar components of nucleoli in a response to DNA damage." Nucleus. 2010;1(4):367-80. [Nucleus][表紙図にして頂きました] [Pubmed]
  • Trembley JH, Tatsumi S, Sakashita E, Loyer P, Slaughter CA, Suzuki H, Endo H, Kidd VJ, Mayeda A. "Activation of pre-mRNA splicing by human RNPS1 is regulated by CK2 phosphorylation." Mol Cell Biol. 2005;25(4):1446-57. [Pubmed]
  • Sakashita E, Tatsumi S, Werner D, Endo H, Mayeda A. "Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo." Mol Cell Biol. 2004;24(3):1174-87. [Pubmed]
  • Tamada H, Sakashita E, Shimazaki K, Ueno E, Hamamoto T, Kagawa Y, Endo H. "cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein." Biochem Biophys Res Commun. 2002;297(1):96-104. [Pubmed]
  • Hayakawa M, Sakashita E, Ueno E, Tominaga S, Hamamoto T, Kagawa Y, Endo H. "Muscle-specific exonic splicing silencer for exon exclusion in human ATP synthase gamma-subunit pre-mRNA." J Biol Chem. 2002;277(9):6974-84. [Pubmed]
  • Kasashima K, Sakashita E, Saito K, Sakamoto H. "Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins." Nucleic Acids Res. 2002;30(20):4519-26. [Pubmed]
  • Kasashima K, Terashima K, Yamamoto K, Sakashita E, Sakamoto H. "Cytoplasmic localization is required for the mammalian ELAV-like protein HuD to induce neuronal differentiation." Genes Cells. 1999;4(11):667-83. [Pubmed]
  • Abe R, Sakashita E, Yamamoto K, Sakamoto H. "Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC." Nucleic Acids Res. 1996;24(24):4895-901. [Pubmed]
  • Sakashita E, Sakamoto H. "Protein-RNA and protein-protein interactions of the Drosophila sex-lethal mediated by its RNA-binding domains." J Biochem (Tokyo). 1996;120(5):1028-33. [Pubmed]
  • Penalva LO, Sakamoto H, Navarro-Sabate A, Sakashita E, Granadino B, Segarra C, Sanchez L. "Regulation of the gene Sex-lethal: a comparative analysis of Drosophila melanogaster and Drosophila subobscura." Genetics. 1996;144(4):1653-64. [Pubmed]
  • Sakashita E, Sakamoto H. "Characterization of RNA binding specificity of the Drosophila sex-lethal protein by in vitro ligand selection." Nucleic Acids Res. 1994;22(20):4082-6. [Pubmed]